overexpression plasmids Search Results


overexpression  (Addgene inc)
92
Addgene inc overexpression
Overexpression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant plasmids for sirt4 overexpression (oe-sirt4)  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd recombinant plasmids for sirt4 overexpression (oe-sirt4)
Recombinant Plasmids For Sirt4 Overexpression (Oe Sirt4), supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ezh2 full-length overexpression plasmid  (Genechem)
90
Genechem ezh2 full-length overexpression plasmid
Ezh2 Full Length Overexpression Plasmid, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dhodh overexpression plasmid  (Shanghai Genechem Ltd)
90
Shanghai Genechem Ltd dhodh overexpression plasmid
Dhodh Overexpression Plasmid, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dhodh overexpression plasmid - by Bioz Stars, 2026-02
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overexpression plasmids  (VectorBuilder GmbH)
90
VectorBuilder GmbH overexpression plasmids
Overexpression Plasmids, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
overexpression plasmids - by Bioz Stars, 2026-02
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14-3-3zeta overexpression plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma 14-3-3zeta overexpression plasmid
14 3 3zeta Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shrna targeting nat10  (Shanghai GenePharma)
90
Shanghai GenePharma shrna targeting nat10
Shrna Targeting Nat10, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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foxl1 overexpression plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma foxl1 overexpression plasmid
Foxl1 Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentivirus encoding the mir-33a precursor (mir-33)  (System Biosciences Inc)
90
System Biosciences Inc lentivirus encoding the mir-33a precursor (mir-33)
Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a <t>lentivirus</t> encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.
Lentivirus Encoding The Mir 33a Precursor (Mir 33), supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
lentivirus encoding the mir-33a precursor (mir-33) - by Bioz Stars, 2026-02
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overexpression plasmids  (Genechem)
90
Genechem overexpression plasmids
Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a <t>lentivirus</t> encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.
Overexpression Plasmids, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
overexpression plasmids - by Bioz Stars, 2026-02
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hur overexpression plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma hur overexpression plasmid
Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a <t>lentivirus</t> encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.
Hur Overexpression Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hur overexpression plasmid/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
hur overexpression plasmid - by Bioz Stars, 2026-02
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irak1 overexpression plasmid  (Molecular Biosciences Inc)
90
Molecular Biosciences Inc irak1 overexpression plasmid
Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a <t>lentivirus</t> encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.
Irak1 Overexpression Plasmid, supplied by Molecular Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak1 overexpression plasmid/product/Molecular Biosciences Inc
Average 90 stars, based on 1 article reviews
irak1 overexpression plasmid - by Bioz Stars, 2026-02
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Image Search Results


Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a lentivirus encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.

Journal: Molecular and Cellular Biology

Article Title: A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression

doi: 10.1128/MCB.01714-12

Figure Lengend Snippet: Both arms of the miR-33 duplex contribute to the posttranscriptional regulation of target gene expression. (A to B) Huh7 cells were transduced with a lentivirus encoding the miR-33a precursor (pre-miR-33) or empty vector control. Transduction efficiency was confirmed 48 h after culturing by measuring GFP expression using fluorescence microscopy (A) and flow cytometry (B). (C) qRT-PCR analysis of miR-33a (top panel) and miR-33a* (bottom panel) in empty vector-transduced or pre-miR-33-transduced Huh7 cells. (D) qRT-PCR analysis of SREBP-2, SREBP-1c, ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in Huh7 cells transduced with pre-miR-33. Values were compared to those from empty vector-transduced cells. (E) qRT-PCR analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, RIP140, and NFYC in pre-miR-33-transduced Huh7 cells transfected with a control inhibitor (CI), inhibitor of miR-33a (Inh-miR-33a), or inhibitor of miR-33a* (Inh-miR-33a*). (F) Western blot analysis of ABCA1, NPC1, CROT, CPT1a, IRS2, SRC1, SRC3, NFYC, and RIP140 in pre-miR-33-transduced Huh7 cells transfected with CI, Inh-miR-33a, or Inh-miR-33a*. Quantification of protein relative to the loading control (HSP90) is shown in the right panel. FS, forward scatter. In panels A to F, the data are means ± SEM and representative of ≥2 experiments in triplicate. *, P ≤ 0.05.

Article Snippet: A lentivirus encoding the miR-33a precursor (miR-33) and empty vector control were obtained from System Biosciences, Inc. (SBI).

Techniques: Targeted Gene Expression, Transduction, Plasmid Preparation, Control, Expressing, Fluorescence, Microscopy, Flow Cytometry, Quantitative RT-PCR, Transfection, Western Blot